Effects of anti- CXCR4 monoclonal antibody on adhesiveness of human acute myelocytic leukemia cell line HL-60 and expression of Bcl-2, Fas proteins.
- Author:
Li WEI
1
;
Pei-Yan KONG
;
Xing-Hua CHEN
;
Xian-Gui PENG
;
Cheng CHANG
;
Dong-Feng ZENG
;
Hong LIU
;
Lin LIU
;
Qing-Yu WANG
;
Yi ZHANG
Author Information
1. Department of Hematology, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, China.
- Publication Type:Journal Article
- MeSH:
Antibodies, Monoclonal;
pharmacology;
Apoptosis;
Cell Adhesion;
Chemokine CXCL12;
Chemokines, CXC;
physiology;
HL-60 Cells;
chemistry;
cytology;
Humans;
Proto-Oncogene Proteins c-bcl-2;
analysis;
Receptors, CXCR4;
physiology;
fas Receptor;
analysis
- From:
Journal of Experimental Hematology
2004;12(4):436-440
- CountryChina
- Language:Chinese
-
Abstract:
To study the importance of chemokine SDF-1 in surviving of acute myelocytic leukemia cells HL-60, the adhesion ability of HL-60 and expression of Bcl-2, Fas protein when SDF-1 activity was blocked by anti-CXCR4 monoclonal antibody (12G5) were compared with those detected before MAb incubation, in this experiment, HL-60 cell were cultured and co-cultured with normal marrow stromal cell. The adhesion rate was detected while the expression of Bcl-2 and Fas proteins were assayed by immunohistochemical technique when SDF-1 activity was inhibited. The results showed that cell adhesion rate of HL-60 decreased while the expression Bcl-2 decreased and Fas increased. It is concluded that inhibition of SDF-1 activity increases cell apoptosis and thus reduces life-span of leukemia cell at certain level.
