Change of Bcl-2, Bax proteins and mitochondrial membrane protein on nitric oxide induced apoptosis in HL-60 cells.
- Author:
Yong-Lie ZHONG
1
;
Lian-Nu QIU
;
Hui-Jun LIN
;
Wen-Song WANG
;
Jian-Guo WU
;
Jian-Dong LIU
Author Information
1. Central Laboratory, Zhejiang Provincial People Hospital, Hangzhou 310014, China. zyl63@mail.hz.zj.cn
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
HL-60 Cells;
Humans;
Membrane Proteins;
analysis;
Mitochondrial Proteins;
analysis;
Nitric Oxide Donors;
pharmacology;
Nitroprusside;
pharmacology;
Proto-Oncogene Proteins c-bcl-2;
analysis;
bcl-2-Associated X Protein
- From:
Journal of Experimental Hematology
2004;12(4):445-449
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the possible mechanisms of nitric oxide (NO)-induced apoptosis in leukemia cell line HL-60, HL-60 cells in vitro were incubated with sodium nitroprusside (SNP), the in situ cell apoptosis quantitatively was assayed by TdT-mediated dUTP nick end labeling (TUNEL), the cell cycle DNA and proteins expression of Bcl-2, Bax, mitochondrial membrane protein (APO2.7) were analyzed by flow cytometry. The results showed that SNP induced HL-60 cell apoptosis in a dosage- and time-dependent manner. After exposure to SNP at the concentration of 1.0 mmol/L for 48 hours, the percentage of apoptosis HL-60 was (42.2 +/- 3.5)% for subG1 and (52.5 +/- 7.6)% for TUNEL respectively, and they are significantly higher than those in control and potassium ferricyanide (PFC) groups as same concentration. During the apoptosis process, it showed a decrease of Bcl-2 protein and an increase of Bax protein and mitochondrial membrane protein in HL-60 cell, proteins of Bcl-2, Bax and mitochondrial membrane were expressed in a dosage- and time-dependent manner too. In conclusion, during the process of SNP induced apoptosis in HL-60 cell, the expression of mitochondrial membrane protein was increased, Bcl-2 and Bax proteins may be important regulators.
