Identification of acute leukemia-specific genes from leukemia recipient/sibling donor pairs by distinguishing study with oligonucleotide microarrays.
- Author:
Yi SUN
1
;
Lu-Jia DONG
;
Fang TIAN
;
Sheng-Qi WANG
;
Zhi-Lin JIA
;
Jian HUANG
;
Ze-Jian CHEN
;
Wu-Ju LI
;
Xi-Lin CHEN
;
Ping ZHU
Author Information
1. Hematopoietic Stem Cell Transplantation Center, Hospital 307, Academy of Military Medical Sciences, Beijing 100039, China.
- Publication Type:Journal Article
- MeSH:
Blood Donors;
DNA-Binding Proteins;
genetics;
Gene Expression Profiling;
Glutathione Transferase;
genetics;
Humans;
Leukemia, Myeloid, Acute;
genetics;
Microtubule Proteins;
genetics;
Milk Proteins;
genetics;
Oligonucleotide Array Sequence Analysis;
Peripheral Blood Stem Cell Transplantation;
Phosphoproteins;
genetics;
Precursor Cell Lymphoblastic Leukemia-Lymphoma;
genetics;
STAT5 Transcription Factor;
Siblings;
Stathmin;
Trans-Activators;
genetics
- From:
Journal of Experimental Hematology
2004;12(4):450-454
- CountryChina
- Language:Chinese
-
Abstract:
To explore differentially expressed genes in leukemia gene expression profile and identify main related genes in acute leukemia, gene expression profiles were analyzed in bone marrow/leucopheresis peripheral blood stem cells samples from 9 acute leukemia patients and their sibling donors with the use of oligonucleotide microarrays. 163 reported leukemia-related genes were involved in the study. The oligonucleotide primers were designed, synthesized and spotted on the chemical-material-coated-glass plates in array. The total RNAs were isolated from nine patients' bone marrow or leucopheresis peripheral blood cells and from nine their sibling donors peripheral blood stem cells treated by G-CSF, then collected by CS-3000 cell selection machine, and were reversely transcribed to cDNAs with the incorporations of fluorescent dUTP. The mixed probes were then hybridized to the oligonucleotide microarray. The results showed that in four patient/donor pairs with B-ALL, 5 up-regulated (RIZ, STK-1, T-cell leukemia/lymphoma 1A, Cbp/p300, Op18) and 1 down-regulated genes (hematopoietic proteoglycan core protein) were identified; In five patient/donor pairs with AML-M(4) and AML-M(5), 6 up-regulated (STAT5B, ligand p62 for the Lck SH2, CST3, LTC4S, myeloid leukemia factor 2 and epb72) and 1 down-regulated genes (CCR5) were identified. In conclusion, on the basis of distinguishing study of specific genetic related recipient/sibling donor pairs, screening leukemia-related genes with oligonucleotide microarrays, a set of 13 up-regulated or down-regulated genes among 163 leukemia-related genes has been identified. The result has further confirmed that above genes play critical role in the molecular mechanism of acute leukemia.
