Detection of minimal residual disease of chronic myeloid leukemia patients after allogeneic stem cell transplantation by combination of STR-PCR with RT-PCR.
- Author:
Xiao-Wen TANG
1
;
Wei WANG
;
De-Pei WU
;
Chang-Geng RUAN
Author Information
1. The First Affiliated Hospital of Suzhou University, Jiangsu Institute of Hematology, Suzhou 215006, China. xwtang1@sina.com
- Publication Type:Journal Article
- MeSH:
Genes, abl;
Hematopoietic Stem Cell Transplantation;
Humans;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive;
diagnosis;
therapy;
Neoplasm, Residual;
Polymerase Chain Reaction;
methods;
Transplantation, Homologous
- From:
Journal of Experimental Hematology
2004;12(4):484-488
- CountryChina
- Language:Chinese
-
Abstract:
In order to evaluate relapse predication ability of STR-PCR combining with qualitative RT-PCR for the bar/abl transcripts to the patient with chronic myeloid leukemia (CML) fulfilled allogeneic stem cell transplantation (allo-HSCT), 24 patients with CML after allo-HSCT were dynamically investigated for MRD, quantitative analysis of donor chimerism was performed by multiplex PCR amplification of STR markers and capillary electrophoresis with fluorescence detection, qualitative detection of bcr/abl transcripts was detected by nested RT-PCR. The results showed that persistent full donor chimerism (DC >/= 95%) was associated with an absence of MRD. All patients with stable MC (90%
