Identification of TPO receptors on central nervous system-a preliminary report.
- Author:
Mo YANG
1
;
Wen-Jie XIA
;
Karen LI
;
Nga-Hin PONG
;
Ki-Wai CHIK
;
Chi-Kong LI
;
Margaret H L NG
;
Ho-Keung NG
;
Kwok-Pui FUNG
;
Tai-Fai FOK
Author Information
1. Department of Paediatrics, The Chinese University of Hong Kong, Hong Kong. yang1091@cuhk.edu.hk
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
drug effects;
Brain Chemistry;
Cell Line;
Cell Proliferation;
drug effects;
Erythropoietin;
pharmacology;
Humans;
Mice;
Neoplasm Proteins;
analysis;
Neurons;
drug effects;
Oncogene Proteins;
analysis;
Proto-Oncogene Proteins;
analysis;
Receptors, Cytokine;
analysis;
Receptors, Thrombopoietin;
Thrombopoietin;
pharmacology
- From:
Journal of Experimental Hematology
2004;12(4):494-497
- CountryChina
- Language:English
-
Abstract:
To identify the expression of thrombopoietin (TPO) receptors (c-mpl) on central nervous system (CNS) and to evaluate the role of TPO on neural cell proliferation and protection, immunohistochemical staining, RT-PCR, MTT, and annexin-V methods were used in this study. The results showed the expression of TPO receptor on human CNS and murine neural cells. C-mpl mRNA was identified in human cerebral hemispheres and cerebellum, and mouse neural cell line C17.2 by RT-PCR. C-mpl was also confirmed in human cerebral hemispheres by immunohistostaining with con-focal microscopy. Furthermore, TPO had a stimulating effect on the growth of in vitro neural cell C17.2 by MTT assay. The anti-apoptotic effect of TPO on C17.2 cells was also demonstrated by staining with annexin-V and PI. In conclusion, the first evidence showed the expression of TPO receptor c-mpl in central nervous system. Moreover, the effect of TPO on neural cell proliferation and anti-apoptosis was also demonstrated on in vitro neural cells.
