Detection of cytokine-secreting T lymphocytes in allogeneic reaction and a preliminary study on its clinical significance.
- Author:
Xiao-Jian MENG
1
;
Mao-Fang LIN
Author Information
1. Department of Hematology, The First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310003, China. mengxiaojian@hotmail.com
- Publication Type:Journal Article
- MeSH:
Acute Disease;
Cytokines;
secretion;
Graft vs Host Disease;
etiology;
Humans;
Interferon-gamma;
secretion;
Interleukin-10;
secretion;
Interleukin-4;
secretion;
T-Lymphocytes;
immunology;
Transplantation, Homologous;
immunology
- From:
Journal of Experimental Hematology
2004;12(4):498-502
- CountryChina
- Language:English
-
Abstract:
In order to explore a new way to study allogeneic reactive T lymphocytes, detection of cytokine-secreting T lymphocytes after allogeneic peripheral blood mononuclear cells (PBMNCs) stimulation and investigation of its clinical significance were performed. A novel cytokine secretion assay (CKSA) was first applied to detect T lymphocytes secreting cytokine including IFN-gamma, IL-4 and IL-10 at single cell level in human mixed lymphocytes reaction. IFN-gamma-secreting T cells from PBMNCs were then evaluated in 2 patients with acute graft versus host disease (aGVHD) after allogeneic bone marrow transplantation. The results showed that compared with IL-4 and IL-10 (which were 0.12 +/- 0.03% and 0.10 +/- 0.03% respectively), a sizable proportion of IFN-gamma-secreting T lymphocytes could be detected (1.12 +/- 0.13)% after allogeneic PBMNCs stimulation. Preliminary results indicated that frequency of IFN-gamma-secreting T lymphocytes correlated with the onset and severity of clinical aGVHD. In conclusion, it is feasible to detect IFN-gamma secreting T lymphocytes after allogeneic PBMNCs stimulation and to apply the CKSA technique for clinical identification of aGVHD.
