Genotyping of ABO loci in para-Bombay type individuals.
- Author:
Jian-Qiang ZENG
1
;
Guang-Ping LUO
Author Information
1. Shenzhen Blood Center, Shenzhen 518035, China. zjq@szbc.org
- Publication Type:Journal Article
- MeSH:
ABO Blood-Group System;
genetics;
Alleles;
Chromosome Mapping;
Female;
Genotype;
Humans;
Male;
Polymerase Chain Reaction;
Sequence Analysis, DNA
- From:
Journal of Experimental Hematology
2004;12(4):513-516
- CountryChina
- Language:Chinese
-
Abstract:
To study the molecular genetic basis of ABO alleles in para-Bombay type individuals, samples from five para-Bombay type individuals identified by serologic tests including absorption-elution tests, saliva neutralizing or inhibitor substances tests, were genotyped by using PCR-SSP based ABO genotyping. Exon 6 and exon 7 at the ABO locus for all 5 samples were sequenced. The results showed that the ABO genotypes of five para-Bombay samples were A102B1, A102B1, A102O1, A102B1, B1O1 respectively, the direct DNA sequencing results were in accordance with the results genotyped by PCR-SSP method, No novel nucleotide mutation was found at the exon 6 and exon 7 of ABO gene. In conclusion, the ABO genotyping assay by PCR-SSP provide a simple, rapid and accurate method for determining the ABO type of para-Bombay cases.
