Establishment of detection method for HCMVpp65 of the blood donors and its application in blood bank.
- Author:
Yan-Chun LIU
1
;
Hon-Li LIU
;
Yi LIU
;
Rong-Cai TANG
;
Da-Xiang SHENG
Author Information
1. Jiangsu Province Blood Center, Nanjing 210042, China.
- Publication Type:Journal Article
- MeSH:
Blood Banks;
Blood Donors;
Female;
Humans;
Immunohistochemistry;
Male;
Phosphoproteins;
blood;
Viral Matrix Proteins;
blood
- From:
Journal of Experimental Hematology
2004;12(4):528-530
- CountryChina
- Language:Chinese
-
Abstract:
To establish method suitable to assay HCMVpp65 of the blood donors in blood bank and to supply safe blood to the patients, the immunocytochemical techniques were used, (6 - 8) x 10(6)/ml cells were counted, 50 x 10(3) cells were detected by light microscope, The results showed that 10 positive samples in 103 samples were found, positive rate was 9.71%, among 10 positive samples, 2 samples were still positive in the second detecting. In conclusion, this method is simple, quick and effective, suitable to detect HCMVpp65 of the blood donors in the blood bank.
