Study on negative regulatory factors in bone marrow mononuclear cells of myelodysplastic syndromes.
- Author:
Hong-Ming HUANG
1
;
Rui-Rong XU
;
Run-Sheng DING
;
De-Yan LU
;
Hong LIU
Author Information
1. Department of Hematology, Affiliated Hospital, Nantong Medical College, Nantong 226001, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Bone Marrow Cells;
metabolism;
Female;
Humans;
Interferon-gamma;
biosynthesis;
Male;
Middle Aged;
Myelodysplastic Syndromes;
metabolism;
pathology;
RNA, Messenger;
analysis;
T-Lymphocyte Subsets;
metabolism;
Tumor Necrosis Factor-alpha;
biosynthesis;
fas Receptor;
genetics
- From:
Journal of Experimental Hematology
2004;12(5):610-614
- CountryChina
- Language:Chinese
-
Abstract:
To explore the difference of negative regulatory factors among T lymphocyte subsets in bone marrow (BM) of myelodysplastic syndromes (MDS) and their relations to apoptotic gene Fas, different lymphocyte subsets in BM were categorized by monoclonal antibodies with 3 color fluorescence using flow cytometry, and the intracellular cytokines such as tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma) were determined following marrow cells culture. Then Fas mRNA of bone marrow mononuclear cells (BMMNC) were examined by RT-PCR. The results showed that TNF-alpha, IFN-gamma levels in BM of MDS both increased, the former produced by cells CD4+CD45RO+, CD8+CD45RO+, the latter by cells CD4+CD45RO+, CD8+CD45RO+, CD8+CD45RA+, in which the cells CD8+CD45RO+ were dominant. Fas mRNA expression had relationship with IFN-gamma produced by T cells but not with TNF-alpha. It is concluded that in hematopoietic microenvironment of MDS, not only the T lymphocyte subsets are in disorder, but also negative regulatory factors secreted by T lymphocyte increase. T lymphocytes play an important role in producing IFN-gamma in patients with MDS.
