Effect of 2-methoxycinnamaldehyde on activity of COX and PGE2 release in cerebral microvascular endothelial cells stimulated by IL-1.
- Author:
Jian-you GUO
1
;
Yuan-xiao YANG
;
Bao-sheng ZHAO
;
Hong-bin LIU
;
Lan-fang LI
;
Yue-Ying MA
;
Shu-ying GUO
;
Hai-ru HUO
;
Ting-liang JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Acrolein; administration & dosage; analogs & derivatives; isolation & purification; pharmacology; Animals; Brain; blood supply; Cells, Cultured; Cyclooxygenase 1; metabolism; Cyclooxygenase 2; metabolism; Dinoprostone; metabolism; Dose-Response Relationship, Drug; Drug Combinations; Drugs, Chinese Herbal; chemistry; isolation & purification; Endothelial Cells; cytology; metabolism; Interleukin-1; antagonists & inhibitors; Male; Microcirculation; cytology; Plants, Medicinal; chemistry; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2006;31(13):1087-1090
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of 2-methoxycinnamaldehyde (isolated from fraction A of Guizhi Tang) on activity of COX and PGE2 release in rat cerebral microvascular endothelial cells (rCMEC) stimulated by IL-1.
METHODrCMEC were cultured, and identified by immunohistochemistry for von Willebrand factor (VIII factor, a marker for all endothelial cells) in cytoplasm of the cells. Different concentrations of 2-methoxycinnamaldehyde were added respectively and incubated for 3 hours, then stimulated for another 12 hours by IL-1. Activities of COX-1 and COX-2 in rCMEC, and production of PGE2 in the conditioned media were measured by ELISA.
RESULTPositive immunostaining for VIII factor was present diffusely in the cytoplasm of > 90% rCMEC. After being exposed to 30 ng x mL(-1) IL, the activity of COX-2 in rCMEC and the production of PGE2 in conditioned media were higher than those of control group, while there was no difference on activity of COX-1 in the two groups. 2-methoxycinnamaldehyde could down-regulate them in concentration-dependently, and significant differences on the activity of COX-2 and amount of PGE2 were showed in 200 microg x mL(-1) concentration.
CONCLUSION2-methoxycinnamaldehyde can affect the PGE2 release in rCMEC induced by IL-1, which might be related with its inhibition on the activity of COX-2.
