Determination of saponin I in extracts of Allium macrostemnon.
- Author:
Hai-feng CHEN
1
;
Nai-li WANG
;
Yi DAI
;
Xin-sheng YAO
Author Information
- Publication Type:Journal Article
- MeSH: Allium; chemistry; Chromatography, High Pressure Liquid; methods; Plant Extracts; chemistry; isolation & purification; Plant Roots; chemistry; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results; Saponins; analysis; Sterols; analysis
- From: China Journal of Chinese Materia Medica 2006;31(12):990-992
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for determination of saponin I in bulb extracts of Allium macrostemon.
METHODThe extract was refluxed by methanol and then filtrated. The filtrate was concentrated and the bioactive saponin was measured by HPLC-RID.
RESULTThe calibration curve was in good linearity over the range of 5-100 microg with the regression equation of Y= 9.35 x 10(4) X - 2.78 x 10(4) (r = 0.9998) and the average recovery was 101.1% (n = 5, RSD 1.6%).
CONCLUSIONThe method is simple, quick and reproducible and can be applied in the quality control of A. macrostemon.
