Study on fingerprints of astragalosides injection by HPLC-ELSD.
- Author:
Jia GUAN
1
;
Zhi-ming BI
;
Ping LI
Author Information
- Publication Type:Journal Article
- MeSH: Astragalus membranaceus; chemistry; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; administration & dosage; chemistry; isolation & purification; Injections; Light; Plants, Medicinal; chemistry; Quality Control; Saponins; administration & dosage; analysis; chemistry; isolation & purification; Scattering, Radiation; Triterpenes; analysis
- From: China Journal of Chinese Materia Medica 2006;31(10):807-809
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for fingerprint determination of the astragalosides injection.
METHODAnalyses were carried out at 25 degrees C on a Zorbax SB-C18 column (4.6 mm x 250 mm,5 microm). Mobile phase A was water, and B was acetonitrile. The analysis followed a linear gradient program. Initial conditions were 15% B; 0 to approximately 65 min, changed to 60% B. The flow rate was 0.8 mL x min(-1). The drift tube temparature of the ELSD was 105 degrees C, and gas flowrate was 2.7 L x min(-1).
RESULTThe RSD of relative retention time of the common peaks in the plant material, the extract and the injection fingerprints was less than 0.1%, and there was good similarity among 10 batches of samples.
CONCLUSIONThe method was reliable and simple which could be used for quality control of the injection.
