HPLC determination of acteoside in Radix Rehmanniae.
- Author:
Hui LI
1
;
Gui-xin CHOU
;
Zheng-tao WANG
;
Zhi-bi HU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Glucosides; analysis; Phenols; analysis; Plant Roots; chemistry; Plants, Medicinal; chemistry; Quality Control; Rehmannia; chemistry; Reproducibility of Results; Sensitivity and Specificity
- From: China Journal of Chinese Materia Medica 2006;31(10):822-824
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for the determination of acteoside in Radix Rehmanniae.
METHODThe chromatographic conditions were as follows: Polaris C18(4.6 mm x 250 mm, 5 microm) column, a mobile phase in gradient mode composed of acetonitrile 0.1% acetic acid solution, a flow rate of 1.0 mL x min(-1), and 334 nm as the detection wavelength.
RESULTActeoside showed good linear relationship at the range of 10-500 microg x mL(-1) (r = 0.9990). The average recovery was 100.1%, RS D 3.7%.
CONCLUSIONThe proposed method promised to be applicable for the quality control of Radix Rehmanniae.
