Estrogenic activity and its mechanism of ethanol extract from black soybean.
- Author:
Qing-wei ZHAO
1
;
Yi-jia LOU
Author Information
- Publication Type:Journal Article
- MeSH: Breast Neoplasms; metabolism; pathology; Cell Line, Tumor; Cell Proliferation; drug effects; Drugs, Chinese Herbal; isolation & purification; pharmacology; Estradiol; analogs & derivatives; pharmacology; Estrogen Receptor Modulators; pharmacology; Female; Humans; Plants, Medicinal; chemistry; RNA, Messenger; biosynthesis; genetics; Receptors, Progesterone; biosynthesis; genetics; Seeds; chemistry; Soybeans; chemistry; Trefoil Factor-1; Tumor Suppressor Proteins; biosynthesis; genetics
- From: China Journal of Chinese Materia Medica 2006;31(10):825-828
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the estrogenic activity and its mechanism of ethanol extract from black soybean (BSE).
METHODThe modified MCF-7 cell proliferation assay was used to evaluate the estrogenic activity of BSE. And the possible mechanisms were addressed using RT-PCR measurements in which pure estrogen receptor antagonist ICI182,780 was employed as a tool.
RESULTThe estrogenic activity of BSE at various concentrations (1-1000 microg x mL(-1)), expressed as proliferative effect (PE) relative to that of solvent control, was examined. The results indicated that, at low concentration range (10-200 microg x mL(-1)), BSE was able to induce MCF-7 cell growth with a maximum at 100 microg x mL(-1). The results of RT-PCR showed that pS2 and PR mRNA could be significantly induced by BSE in MCF-7 cells, which was relative to the concentration of BSE. Moreover, the specific estrogen receptor antagonist, ICI182,780 could block these reactions.
CONCLUSIONEthanol extract of black soybean can stimulate the growth of MCF-7 cells and increase the expression of estrogen receptor-responsive gene, suggesting that the estrogenic effects of BSE are mediated by the estrogen receptor.
