Study on time-effect and dose-effect of serum containing Chinese herbal medicine.
- Author:
Jian-xun LIU
1
;
Xiao HAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Hypoxia; Cells, Cultured; Corydalis; chemistry; Dose-Response Relationship, Drug; Drug Combinations; Drugs, Chinese Herbal; administration & dosage; isolation & purification; pharmacokinetics; Female; L-Lactate Dehydrogenase; metabolism; Male; Myocytes, Cardiac; cytology; metabolism; Panax; chemistry; Plants, Medicinal; chemistry; Rats; Rats, Sprague-Dawley; Salvia miltiorrhiza; chemistry; Serum; Time
- From: China Journal of Chinese Materia Medica 2006;31(10):829-831
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the time-effect, dose-effect of serum containing Shuangshentongguan (SSTG), a formula composed by active fraction of Chinese medicine.
METHODSerum containing SSTG was obtained at different time after perorally administrated with different dose of SSTG. The cardiomyocytes were deprived of oxygen and glucose to mimic hypoxia reoxygenation injury and were treated by serum containing SSTG when reoxygenation. LDH content in supernatant was detected after reoxygenation. LDH release suppression rate was used to study the time-effect and dose-effect of serum containing SSTG.
RESULTThe suppression rate of LDH release was not satisfied in serum containing high dose of SSTG. There was no significant dose-effect relationship between them. The suppression rate of LDH release was also different in drug serum taken at different time after SSTG treatment.
CONCLUSIONIt was not the case that the higher the dose given to animal, the better the pharmacological effects.
