Determination of akebia saponin D in root of Dipacus asperoides by HPLC.
- Author:
Hong-gen TAN
1
;
Sheng LIN
;
Qi-wei ZHANG
;
Li JI
Author Information
- Publication Type:Journal Article
- MeSH: China; Chromatography, High Pressure Liquid; Dipsacaceae; chemistry; Ecosystem; Plant Roots; chemistry; Plants, Medicinal; chemistry; Quality Control; Saponins; analysis
- From: China Journal of Chinese Materia Medica 2006;31(9):726-739
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a quantitative method for the determination of akebia saponin D in root of Dipacus asperoides and provide scientific basis for quality control of D. asperoides.
METHODAn IPLC analytical method was established using Kromasil ODS column with acetonitrile-water (30:70) as mobile phase and the detection wavelength was 212 nm.
RESULTThe akebia saponin D in root extract were well separated by this method. The linear range is between 0.582 5-9.32 microg, r = 0.9999. The average recovery and RSD of repeatability are 100.3%, 2.3%, respectively.
CONCLUSIONThis method is simple, accurate and reliable by evaluating the method validation data and can be used for the quality control of D. asperoides and its preparations.
