Study on determination of entrapment efficiency of sinomenine liposomes.
- Author:
Li-ling ZHOU
1
;
Yan WANG
;
Qing-fei LIU
;
Jia-jun LING
Author Information
- Publication Type:Journal Article
- MeSH: Dextrans; Drug Carriers; Drug Delivery Systems; Filtration; methods; Liposomes; Morphinans; administration & dosage; analysis; isolation & purification; Sinomenium; chemistry; Technology, Pharmaceutical; methods
- From: China Journal of Chinese Materia Medica 2006;31(9):731-734
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an HPLC method for the determination of entrapment efficiency of sinomenine liposomes.
METHODThe liposomes and dissociated drugs were separated by sephadex filtration, mini-column centrifugation and dialysis. The methodology study and the optimization of determining condition were carried out at the same time.
RESULTSephadex filtration could effectively separate the sinomenine liposomes from dissociated sinomenine. The column recovery was 98.8%, the average entrapment efficiency of three tests was64.9%, RSD 2.67%.
CONCLUSIONThe method was simple, exact, and had a good reappearance. It can be used to examine the entrapment efficiency of sinomenine liposomes.
