Determination of two polyacetylenes in Herba Lobeliae Chinensis by HPLC analysis.
- Author:
Chun-feng QIAO
1
;
Zhen-dan HE
;
Quan-bin HAN
;
Jing-zheng SONG
;
Hong-xi XU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Lobelia; chemistry; Molecular Structure; Plants, Medicinal; chemistry; Polyacetylenes; analysis; chemistry; Quality Control; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2006;31(9):744-746
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for determination of two polyacetylenes, lobetyolin and lobetyolinin, in Herba Lobeliae Chinensis.
METHODC18 column was used with the mobile phase consisted of acetonitrile and water. Linear gradient elution from 10% to 40% acetonitrile in 25 min was applied, at the flow rate of 1.0 mL x min(-1), the detection wavelength was at 267 nm.
RESULTLower contents of lobetyolin and lobtyolinin were found in collected samples of Herba Lobeliae Chinensis. The highest amounts of lobetyolin and lobetyolinin were found to be 0.461 and 0.436 mg x g(-1) in a sample procured from Hong Kong. However, there were no lobetyolin and lobetyolinin in some of the samples.
CONCLUSIONA simple and effective HPLC method to analyze the two polyacetylenes in Herba Lobeliae Chinensis was established. It could be applied for the quality control of this herb.
