Determination of danshensu and protocatechuic aldehyde in Guanxinning injection powder by RP-HPLC.
- Author:
Xiao-Rui GUO
1
;
Wen-Ting ZHANG
;
Xiao-Hui CHEN
;
Kai-Shun BI
Author Information
- Publication Type:Journal Article
- MeSH: Benzaldehydes; analysis; Catechols; analysis; Chromatography, High Pressure Liquid; methods; Drug Combinations; Drugs, Chinese Herbal; administration & dosage; chemistry; isolation & purification; Injections; Lactates; analysis; Ligusticum; chemistry; Plants, Medicinal; chemistry; Powders; Quality Control; Reproducibility of Results; Salvia miltiorrhiza; chemistry
- From: China Journal of Chinese Materia Medica 2006;31(16):1328-1330
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEAn RP-HPLC procedure was established for the determination of danshensu and protocatechuic aldehyde in Guanxinning injection powder.
METHODAn RP-HPLC analytical procedure was developed using Hypersil ODS2 C18 column (4.6 mm x 250 mm, 5 microm) with methanol-0.5% glaceal acetic acid (4.5:95.5) as mobile phase, and a wavelength of 280 nm for UV detection.
RESULTThe linear range was 3.004-45.06 microg x m(L-1) (r = 0.999 5, n = 6) for danshensu, and 0.300-4.509 microg x mL(-1) (r = 0.999 3, n = 6) for protocatechuic aldehyde. The average recoveries were 99.1% and 97.9%, respectively.
CONCLUSIONThe method was stable, accurate, and reproducible, and can be used for the quality control of Guanxinning injection powder.
