Determination of papaverine in Qiangli Pipalu by HPLC.
- Author:
Wen-Jie QIN
1
;
Kai MA
;
Han GAO
;
Gang-Li WANG
;
Rui-Chao LIN
Author Information
- Publication Type:Journal Article
- MeSH: Antitussive Agents; chemistry; pharmacology; Chromatography, High Pressure Liquid; methods; Drug Combinations; Drugs, Chinese Herbal; chemistry; isolation & purification; pharmacology; Eriobotrya; chemistry; Expectorants; chemistry; pharmacology; Papaver; chemistry; Papaverine; analysis; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2006;31(16):1330-1332
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for the determination of the papaverine content in Qiangli Pibalu by HPLC.
METHODA C18 column with a solvent system of acetonitrile-0.02 mol x L(-1) sodium dihydrogen phosphate (0.2% triethylamine, phosphoric acid, at pH 3) (25:75) and UV detection 240 nm were used. The flow rate was 1.0 mL x min(-1). The column temperature was maintained at 40 degrees C.
RESULTThere was a good linear relationship between the absorption value and the concentration in the range of 0.020 2-0.100 5 microg for papaverine. The average recovery rates were 99.1% (RSD 2.3%).
CONCLUSIONThe method is simple, accurate and can be used to determine the contents in Qiangli Pibalu.
