Differentiation of HL-60 cells induced by realgar nano-particles.
- Author:
Li-Yun LUO
1
;
Tian-Lan ZHANG
;
Kui WANG
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; administration & dosage; pharmacology; Arsenicals; administration & dosage; pharmacology; CD11b Antigen; metabolism; Cell Differentiation; drug effects; Cell Proliferation; drug effects; G1 Phase; drug effects; HL-60 Cells; Humans; Lipopolysaccharide Receptors; metabolism; Materia Medica; administration & dosage; pharmacology; Nanoparticles; Sulfides; administration & dosage; pharmacology
- From: China Journal of Chinese Materia Medica 2006;31(16):1343-1346
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the proliferation inhibition and the differentiation effects of realgar (As4S4) nano-particles on human acute myeloid leukemia cell line HL-60.
METHODCell viability was determined by MTT and PI-stained cell cycle assays. The realgar induced morphological changes on cells were examined after Wright-Giemsa staining. The cell differentiation was evaluated with NBT and specific cell surface antigen (CD11b and CD14) expression assays.
RESULTHL-60 cells exhibited obvious morphological features of differentiation after the realgar treatment. A 24 h incubation of the cells with 0.25-1.0 micromol x L(-1) realgar caused a great increase in NBT reduction ability. The expressions of CD11b and CD14 were augmented in cells treated with 0.50 micromol x L(-1) realgar for 48 h, and cell cycles were arrested in G1 phase.
CONCLUSIONLow dose realgar induces differentiation in human acute myeloid leukemia cell line HL-60.
