Radiofrequency ablation can reverse the abnormal circulating microRNA expression changes in patients with atrial fibrillation
10.3760/cma.j.issn.0253-3758.2015.12.009
- VernacularTitle:心房颤动射频消融手术可逆转微小RNA的调控异常
- Author:
Yuxia CUI
1
;
Ying SU
;
Long LI
;
Sheng ZHAO
;
Jing NAN
;
Yunan YUE
;
Shuixiang YANG
Author Information
1. 100038,首都医科大学附属北京世纪坛医院心内科
- Keywords:
Atrial fibrillation;
MicroRNAs;
Genomics;
Catheter ablation
- From:
Chinese Journal of Cardiology
2015;43(12):1051-1056
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the change of circulating microRNAs (miRNAs),regulatory mechanism in patients with atrial fibrillation (AF) before and after radiofrequency ablation (RFA).Methods From Jauuary 2011 to December 2013,peripheral blood samples were taken from 30 AF patients (10 paroxysmal,10 persistent and 10 permanent AF) before and 3 months after RFA.The total RNA was extracted and hybridized with the miRNA chips,and the differential expression of miRNA and clustering analysis in whole genome were made with Volcano Plot and tMEV software respectively,and validated by real-time PCR.The target gene analysis of miRNAs was predicted through the Mirbase,Miranda and Targetscan databases.Results were compared with those from 10 heahhy subjeets (control group).Results Compared with control group,the expressions of 25 miRNAs were down-regulated before RFA and up-regulated after RFA in AF group,while other 40 miRNAs expression ehauged in the opposite way;among them,the expressions of 7 miRNAs including miR-199a-3p/miR-199b-3p were down-regulated ＞ 1.5-fold before RFA and up-regulated ＞ 100-fold after RFA;oppositely,6 miRNAs including miR-BART8-3p were up-regulated ＞ 1.5-fold before RFA and down-regulated ＞ 10-fold after RFA.Interestingly,6 miRNAs including miR-30b-5p,which were involved in AF-related electrical aud structural remodeling,were down-regulated ＞5-fold before RFA,but up-regulated ＞ 50-fold after RFA.Four miRNAs including miR-377-5p,which were involved in the regulation of CACNA1C ICa L channel protein,were different before and after RFA.Conclusion miRNAs regulate the occurrence and development of AF.RFA can change the expression of miRNAs in AF patients,which may be important for reversing the electrical and structural remodeling and maintaining sinus rhythm after RFA.miRNAs,such as miR-30b-5p,miR-377-5p and miR-199a-3p/miR-199b-3p etc.,might become the target markers for early diagnosis and intervention of AF in future.
