Calpain-2 and calcineurin protein expression in right atrial appendages from patients underwent valve replacement with or without atrial fibrillation.
- Author:
Zhang QING
;
Wang JIANCHUN
;
Zhao YONG
;
Zhou NANNAN
;
Li CONG
;
Wang LIJUAN
;
Sun XIA
- Publication Type:Journal Article
- MeSH: Atrial Appendage; Atrial Fibrillation; Blotting, Western; Calcineurin; Calpain; Heart Valve Diseases; Humans; Up-Regulation
- From: Chinese Journal of Cardiology 2015;43(8):705-708
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between the protein expression of calpain-2 and calcineurin (CaN) and atrial fibrillation (AF) in patient with valvular heart disease (VHD).
METHODSA total of 40 patients who underwent valve replacement surgery in our hospital from March 2013 to March 2014, right atrial appendages were excised during operation and patients were divided into sinus rhythm (SR) group (n = 17) and AF group (n = 23). The protein expression of calpain-2 and the α-isoform of CaN catalytic subunit (CnA) in the right atrial appendages were determined by Western blot.
RESULTSThe protein levels of the full-length CnAa (60,000), the 45,000 fragment of CnAa without autoinhibitory domain, and calpain-2 were significantly upregulated in the AF group compared to the SR group (1.25 ± 0.51 vs. 0.76 ± 0.37, 1.08 ± 0.37 vs. 0.76 ± 0.25, and 0.82 ± 0.44 vs. 0.51 ± 0.19, respectively, all P < 0.05).
CONCLUSIONActivated calpain-2-CaN signal pathway might be involved in the pathogenesis of AF.