Efficient polyhydroxybutyrate production from cheap resources by recombinant Escherichia coli.
- Author:
Guoqing WEI
1
;
Quan CHEN
;
Zhen KANG
;
Qingsheng QI
Author Information
1. State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
- Publication Type:Journal Article
- MeSH:
Escherichia coli;
genetics;
metabolism;
Fermentation;
Genetic Vectors;
genetics;
Hydroxybutyrates;
metabolism;
Metabolic Engineering;
methods;
Mutation;
Polyesters;
metabolism
- From:
Chinese Journal of Biotechnology
2010;26(9):1257-1262
- CountryChina
- Language:Chinese
-
Abstract:
Based on the fermentation analysis of Escherichia coli strains and cheap renewable resources suitable for polyhydroxybutyrate (PHB) production, we constructed a ptsG mutant of Escherichia coli DH5alpha. Application of E. coli DH5alpha mutant together with stress-induced system, we could produce PHB efficiently from cheap renewable sugar mixture by the simultaneous consumption of different sugars. Batch fermentation at lab scale (5 liter) showed that E. coli DH5alpha deltaptsG/pQKZ103 produced PHB from sugar mixture up to 84.6% of cell dry weight in 32 hours; meanwhile, the cell dry weight reached 8.24 g/L.
