Recycle of spent cells from anaerobic succinate fermentation.
- Author:
Xuefei BAI
1
;
Kequan CHEN
;
Guizi YE
;
Xiumei HUANG
;
Jian LI
;
Min JIANG
Author Information
1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China.
- Publication Type:Journal Article
- MeSH:
Actinobacillus;
metabolism;
Anaerobiosis;
Culture Media;
pharmacology;
Fermentation;
Industrial Waste;
Succinic Acid;
metabolism
- From:
Chinese Journal of Biotechnology
2010;26(9):1276-1280
- CountryChina
- Language:Chinese
-
Abstract:
Spent cells recovered from anaerobic fermentation by Actinobacillus succinogenes were used as nitrogen source for succinic acid production. Three methods were investigated for cell wall-breaking. The results showed that enzymatic hydrolysis was more effective for higher succinic acid yield. When the enzymatic hydrolysate of spent cells was added to reach a total nitrogen concentration 1.11 g/L (equivalent to 10 g/L yeast extract), the succinic acid concentration was 42.0 g/L, but it increased slightly when enhancing the level of enzymatic hydrolysate. However, when 5 g/L yeast extract was supplemented with the enzymatic hydrolysate of spent cells, the succinic acid concentration reached 75.5 g/L after 36 hours and, the succinic acid productivity was 2.10 g/(L x h), which increased by 66.7% compared with the fermentation using 10 g/L yeast extract. Therefore, enzymatic hydrolysate of spent cells could replace 50% yeast extract in the original medium for succinic acid production.
