Metabolic flux analysis of L-serine synthesis by Corynebacterium glutamicum SYPS-062.
- Author:
Xiaomei ZHANG
1
;
Wenfang DOU
;
Hongyu XU
;
Zhenghong XU
Author Information
1. Laboratory of Pharmaceutical Engineering, School of Medicine and Pharmaceutics, Jiangnan University, Wuxi 214122, China.
- Publication Type:Journal Article
- MeSH:
Citric Acid Cycle;
physiology;
Corynebacterium glutamicum;
metabolism;
Fermentation;
Folic Acid;
pharmacology;
Serine;
biosynthesis;
Vitamin B 12;
pharmacology
- From:
Chinese Journal of Biotechnology
2010;26(10):1363-1371
- CountryChina
- Language:Chinese
-
Abstract:
Corynebacterium glutamicum SYPS-062 was an L-serine producing strain stored at our lab and could produce L-serine directly from sugar. We studied the effects of cofactors in one carbon unit metabolism-folate and VB12 on the cell growth, sucrose consumption and L-serine production by SYPS-062. In the same time, the metabolic flux distribution was determined in different conditions. The supplementation of folate or VB12 enhanced the cell growth, energy synthesis, and finally increased the flux of pentose phosphate pathway (HMP), whereas the carbon flux to L-serine was decreased. The addition of VB12 not only increased the ratio of L-serine synthesis pathway on G3P joint, but also caused the insufficiency of tricarboxylic acid cycle (TCA) flux, which needed more anaplerotic reaction flux to replenish TCA cycle, that was an important limiting factor for the further increasing of the L-serine productivity.
