Mechanism of DNA transformation based on mineral nanofibers and method improvement.
- Author:
Haidong TAN
1
;
Lei WANG
;
Jintao LIN
;
Zongbao ZHAO
Author Information
1. Biotechnology Division, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
- Publication Type:Journal Article
- MeSH:
DNA, Bacterial;
chemistry;
genetics;
Electroporation;
methods;
Magnesium Silicates;
chemistry;
Mineral Fibers;
Nanofibers;
chemistry;
Transformation, Bacterial
- From:
Chinese Journal of Biotechnology
2010;26(10):1379-1384
- CountryChina
- Language:Chinese
-
Abstract:
Sepiolite--an inexpensive, resourceful, fibrous yet inoffensive mineral--made DNA transformation rapid, simple and efficient but the mechanism for DNA transformation was still unclear. Through RNA competition test, we proposed the different transforming mechanisms from the previous report. Meanwhile, we optimized the transforming method and could transfer a colony stored at 4 degrees C for a month with plasmid through sepiolite fibers. The cells could be transformed well without competent cells preparation or incubation process. In sum, this was a novel potential transforming method, which could be explored further if the chemical method and electroporation could not be used.
