Substrate specificity of carotenoid 3',4'-desaturase from Deinococcus radiodurans.
- Author:
Zongtao SUN
1
;
Bing TIAN
;
Shaochuan SHEN
;
Yuejin HU
Author Information
1. Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310029, China.
- Publication Type:Journal Article
- MeSH:
Carotenoids;
biosynthesis;
genetics;
metabolism;
Deinococcus;
enzymology;
genetics;
Escherichia coli;
genetics;
metabolism;
Oxidoreductases;
metabolism;
Substrate Specificity
- From:
Chinese Journal of Biotechnology
2010;26(10):1451-1455
- CountryChina
- Language:Chinese
-
Abstract:
To examine the substrate specificity of carotenoid 3',4'-desaturase (DR2250) from Deinococcus radiodurans, we amplified the dr2250 gene by using PCR methods. The PCR products were digested by Hind III-BamH I and ligated into the vector pUC19, yielding recombinant vector pUC-CRTD. We analyzed the carotenoids of E. coli transformants containing pACCRT-EBI(Eu) and (or) pRK-CRTC and (or) pUC-CRTD. Our results demonstrated that DR2250 had substrate specificity on the carotenoids with hydroxyl group at C1 (1').
