Cyclosporin A Inhibits Albumin Synthesis in Huh7 Cells.
10.3904/kjim.2011.26.3.314
- Author:
Youn Joo JEON
1
;
Yong Soo KIM
Author Information
1. Bupyoung Internal Medicine Clinic, Incheon, Korea.
- Publication Type:Original Article
- Keywords:
Cyclosporin A;
Albumins;
Kidney transplantation;
Hypoalbuminemia
- MeSH:
Blotting, Northern;
Blotting, Western;
Carcinoma, Hepatocellular/genetics/*metabolism;
Cell Line, Tumor;
Cell Survival/drug effects;
Culture Media, Conditioned/metabolism;
Cyclosporine/*pharmacology/toxicity;
Dose-Response Relationship, Drug;
Enzyme-Linked Immunosorbent Assay;
Gene Expression Regulation, Neoplastic/drug effects;
Humans;
Hypoalbuminemia/chemically induced/metabolism;
Immunosuppressive Agents/*pharmacology/toxicity;
Liver Neoplasms/genetics/*metabolism;
RNA, Messenger/metabolism;
Serum Albumin/genetics/*metabolism;
Time Factors
- From:The Korean Journal of Internal Medicine
2011;26(3):314-319
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/AIMS: Hypoalbuminemia occurs frequently in renal transplant recipients immediately after renal transplantation. We studied the regulation of hepatic albumin synthesis by cyclosporin A (CsA) in Huh7 cells. METHODS: Huh7 cells were incubated with various concentrations of CsA for 4, 8, 16, and 24 hours. Albumin was measured in Huh7 cell-conditioned medium by sandwich enzyme-linked immunosorbent assay and Western blot. Albumin mRNA expression was analyzed by Northern blotting in CsA-treated cells. RESULTS: CsA (10(-7)-10(-4) M) inhibited albumin synthesis in Huh7 cells in a dose- dependent manner. A Western blot analysis for albumin in the conditioned medium released from CsA-treated (10(-7)-10(-5) M) cells also showed significant inhibition of albumin synthesis in a dose-dependent manner. Vehicle (olive oil) did not affect albumin synthesis. In contrast, a Northern blot analysis revealed no inhibition of albumin mRNA expression by CsA at any time point from 1-24 hours, indicating that the inhibition of albumin synthesis occurred at the translational level. CONCLUSIONS: Our results suggest that inhibition of hepatic albumin synthesis by high dose CsA contributes to the hypoalbuminemia in renal transplant recipients.
