Effects of different neutralizing agents on succinate production by Actinobacillus succinogenes NJ113.
- Author:
Zhuona YANG
1
;
Min JIANG
;
Jian LI
;
Xiaojiang FANG
;
Guizi YE
;
Xuefei BAI
;
Xiaoyu ZHENG
;
Ping WEI
Author Information
1. State Key Laboratory of Materials-Oriented Chemical Engineering, College ofBiotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China.
- Publication Type:Journal Article
- MeSH:
Actinobacillus;
genetics;
metabolism;
Fermentation;
Hydrogen-Ion Concentration;
Industrial Microbiology;
Magnesium Hydroxide;
pharmacology;
Sodium Hydroxide;
pharmacology;
Succinic Acid;
metabolism
- From:
Chinese Journal of Biotechnology
2010;26(11):1500-1506
- CountryChina
- Language:Chinese
-
Abstract:
Different neutralizing agents were used as pH controller to investigate their effects on the growth and succinic acid production of Actinobacillus succinogenes NJ113. The fermentation results showed that Ca(OH)2, CaCO3 and NH4OH were not suitable for succinic acid production by A. succinogenes NJ113 because of their negative effects on cell growth. When Na-base was used, cells would flocculate and lump, and due to the sodium ion concentration reaching to a high level, OD660 dropped sharply after 12 h of fermentation. Mg-base was better because there was no significant inhibition by magnesium ion. Two combined neutralizing agents were used to maintain pH level, one with NaOH and Mg(OH)2 while the other with Na2CO3 and Mg(OH)2. The optimum ratios of the combined neutralizing agents were both 1:1 (g:g) when using 100 g/L glucose. When NaOH and Mg(OH)2 were chosen with the ratio of 1:1(g:g), 69.8 g/L of the succinic acid and 74.5% of the yield was obtained.
