Isolation, cultivation and identification of adipose-derived stem cell in bovines.
- Author:
Yu REN
1
;
Haiqing WU
;
Yuzhen MA
;
Ming CANG
;
Rui WANG
;
Dongjun LIU
Author Information
1. Key Laboratory of Ministry of Education of China for Mammal Reproduction Biology and Biotechnology, Inner Mongolia University, Huhhot 010021, China.
- Publication Type:Journal Article
- MeSH:
Adipose Tissue;
cytology;
Animals;
Cattle;
Cell Differentiation;
physiology;
Cell Separation;
Cells, Cultured;
Mesenchymal Stromal Cells;
cytology;
Tissue Engineering;
methods
- From:
Chinese Journal of Biotechnology
2010;26(12):1645-1651
- CountryChina
- Language:Chinese
-
Abstract:
To obtain bovine adipose-derived stem cells (ADSCs), bovine ADSCs were digested in collagenase type I solution. The growth curve of ADSCs was checked by cell counting. Chromosome analysis was checked. The molecular markers of ADSCs were detected with immunofluorescence staining. The morphology of ADSCs was identical to fibroblast like and the cells showed active proliferative ability. Vimentin, CD49d and CD13 antigens were detected, but CD34 antigen was negative. Alkaline phosphatase activity was greater in ADSCs during calcification, and Alizarin Red staining was positive. Lipid droplets were apparent around cells during adipogenesis, and Oil Red-O staining was positive. The results demonstrated that ADSCs could be used as seed cells for tissue engineering due to the simple isolation, differentiation and stable and active growth.
