Quantitative analysis of telomerase reverse transcriptase gene expression in goat reprogramming cells.
- Author:
Shujin ZHANG
1
;
Shuyan MENG
;
Lei LEI
;
Xiang CHENG
;
Huayan WANG
Author Information
1. Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Veterinary Medicine, Shaanxi Center for Stem Cell Engineering and Technology, Northwest A&F University, Yangling 712100, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cellular Reprogramming;
Fibroblasts;
cytology;
Gene Expression Regulation;
Goats;
Induced Pluripotent Stem Cells;
cytology;
metabolism;
RNA-Directed DNA Polymerase;
genetics;
Telomerase;
metabolism
- From:
Chinese Journal of Biotechnology
2010;26(12):1660-1667
- CountryChina
- Language:Chinese
-
Abstract:
Currently, animal somatic cell reprogramming into the induced pluripotent stem cell (iPS) is one of the hottest research target in the field of cell biology. We focused on the analysis of telomerase reverse transcriptase (TERT) gene expression during goat somatic fibroblasts reprogramming, and investigated the relationship between the expression of TERT and the pluripotency of reprogrammed cells. RNA samples of fetal tissues isolated from Guanzhong milk goat fetus, and the induced goat reprogramming cell clones were used to determine the relative expression levels of TERT by the real-time RT-PCR method. Goat embryonic fibroblasts (GEF) collected from the Guanzhong milk goat with normal karyotype were induced by 4 transcription factors to become reprogramming cells. The expression of TERT in reprogramming cells was detected by Real-time RT-PCR. The results showed that the expression of TERT in testis tissue was higher than that in epithelial tissues (P < 0.01). The expression level of TERT was higher in AP staining positive cells than that in AP staining negative cells (P < 0.01). This result indicated that TERT activity played an important role in cell reprogramming.
