Preparation and application of goat deltafosB gene expression product antibody.
- Author:
Huiling ZHENG
1
;
Zhenzhen ZHU
;
Junhui AN
;
Zhenyu YANG
;
Ruifang XING
;
Linhui YAN
Author Information
1. College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China. Zheng.huiling@yahoo.com
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Monoclonal;
biosynthesis;
Cloning, Molecular;
Escherichia coli;
genetics;
metabolism;
Gene Expression;
Goats;
Proto-Oncogene Proteins c-fos;
biosynthesis;
genetics;
immunology;
Rabbits;
Recombinant Proteins;
biosynthesis;
genetics;
immunology
- From:
Chinese Journal of Biotechnology
2010;26(12):1704-1709
- CountryChina
- Language:Chinese
-
Abstract:
deltaFosB, a naturally occurring truncated isform of fosB gene, existed in many tissues stably and played an important role in formation and differentiation of adipocyte and osteoblast. deltaFosB may be related to the metabolism of calcium in bone and mammary gland and regulate the signal pathway of calcium transfer from bone to mammary gland. We first sub-cloned deltafosB gene of goat into the vector pET32a to construct prokaryotic expression vector pET32a-deltafosB. Then we induced for deltafosB gene expression efficiently by IPTG. Finally we immunized the adult rabbits with purified recombinant deltaFosB to prepare rabbit anti-goat deltaFosB polyclonal antibody. iELISA analysis showed the antibody with the titer of 1:51 200, and Western blotting result showed that the antibody could specifically detect the deltaFosB protein expressed in prokaryotic cell and HEK-293 cell, respectively. Further Western blotting assay showed that deltaFosB expressed in various tissues of goat in vivo.
