Analysis of porcine preadipocytes differentiation by atomic force microscope.
- Author:
Shengpu LI
1
;
Ruyi SHI
;
Qiulan WANG
;
Mu WANG
;
Rui GAN
;
Jie PAN
;
Jiye CAI
;
Shouquan ZHANG
Author Information
1. Department of Chemistry, Jinan University, Guangzhou 510632, China.
- Publication Type:Journal Article
- MeSH:
Adipocytes;
cytology;
ultrastructure;
Adipogenesis;
Animals;
Cell Differentiation;
physiology;
Cells, Cultured;
Microscopy, Atomic Force;
Swine
- From:
Chinese Journal of Biotechnology
2011;27(1):124-130
- CountryChina
- Language:Chinese
-
Abstract:
Abnormal changes during fat formation are closely related to the prevalence of many diseases. In order to understand the formation mechanism of fat, we used atomic force microscopy (AFM) to characterize the morphology and mechanical properties of porcine preadipocytes during the differentiation. Preadipocytes and adipocytes were different morphologically. The surface roughness of adipocytes was less than preadipocytes by detection of the ultrastructure. The mechanical properties of preadipocytes were changed during differentiation with AFM-based force spectroscopy. Preadipocytes were 20% higher than adipocytes in the adhesion force, stiffness and Young's modulus. Therefore, AFM analysis of membrane changes related to adipocytes formation provided quantitative data in the nanometer level for further studying the formation mechanism of the adipocytes.
