Operating conditions for the rapid mutation of the oleaginous yeast by atmospheric and room temperature plasmas and the characteristics of the mutants.
- Author:
Lihua JIN
1
;
Mingyue FANG
;
Chong ZHANG
;
Peixia JIANG
;
Nan GE
;
Heping LI
;
Xinhui XING
;
Chengyu BAO
Author Information
1. Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
- Publication Type:Journal Article
- MeSH:
Atmosphere;
Biofuels;
Culture Media;
Fermentation;
Glucose;
pharmacology;
Lipids;
biosynthesis;
Mutation;
Peptones;
pharmacology;
Temperature;
Yeasts;
genetics;
growth & development;
metabolism
- From:
Chinese Journal of Biotechnology
2011;27(3):461-467
- CountryChina
- Language:Chinese
-
Abstract:
To obtain oleaginous yeast mutants with improved lipid production and growth rates, an atmospheric and room temperature plasma (ARTP) jet was used with a 96-well plate for high throughput screening. Mutants with changes in growth rates and lipid contents were obtained. At a lethality rate of 99%, the positive mutation rate of the yeast cells was 27.2% evaluated by the growth rates of the mutants and the comparison with the wild strain. The fermentation in a medium composed of yeast extract (10 g/L), peptone (10 g/L) and D-glucose (20 g/L) resulted in the lipid yield of the mutant (C4) with 4.07% (W/W) compared with that of the wild strain (1.87%).
