Screening of high lipid production Trichosporon fermentans mutants by transposon tagging mTn-lacZ/leu2 insertion.
- Author:
Andong SONG
1
;
Yubo LIU
;
Hui XIE
;
Fengqin WANG
;
Xiaoming BAO
Author Information
1. Life Science College, Henan Agricultural University, Zhengzhou 450002, China. song1666@126.com
- Publication Type:Journal Article
- MeSH:
3-Isopropylmalate Dehydrogenase;
genetics;
Biofuels;
DNA Transposable Elements;
genetics;
Fermentation;
Lac Operon;
genetics;
Lipids;
biosynthesis;
Mutagenesis, Insertional;
Mutation;
Plant Stems;
metabolism;
Saccharomyces cerevisiae Proteins;
genetics;
Trichosporon;
genetics;
metabolism;
Zea mays;
metabolism
- From:
Chinese Journal of Biotechnology
2011;27(3):468-474
- CountryChina
- Language:Chinese
-
Abstract:
To improve microbial lipid production, we inserted mTn-lacZ/leu2 into Trichosporon fermentans 2.1368-Leu(-) to obtain high lipid production mutants. By observing the LacZ chromogenic change, the positive reaction between Cerulenin (inhibitor of fatty acid synthase) and phosphate vanillin, a higher lipid-producing mutant 2.1368-Leu(-)-7 grown on corn-stalk hydrolysate was obtained. The lipid content of this mutant reached 38.30% (8.97% higher than that of the control) and the lipid production rate was 8.35% (20.63% higher than that of the control). The rate of sugar utilization was 77%, meaning that 100 g corn-stalk could be converted to 8.32 g lipid. The study provided an effective method for microbial lipid production by using cheap raw materials for biodiesel.
