Comparison of 2,3-butanediol production by several strains and optimization of the fermentation medium.
- Author:
Yuanquan SONG
1
;
Ruchun WU
;
Yunzhen XU
;
Ming FAN
;
Dehua LIU
Author Information
1. Institute of Applied Chemistry, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
- Publication Type:Journal Article
- MeSH:
Butylene Glycols;
metabolism;
Culture Media;
chemistry;
Fermentation;
Gene Knockout Techniques;
Glucose;
metabolism;
Klebsiella pneumoniae;
classification;
genetics;
growth & development;
metabolism
- From:
Chinese Journal of Biotechnology
2011;27(3):489-492
- CountryChina
- Language:Chinese
-
Abstract:
Five Klebsiella pneumonia strains (including two strains whose genes for lactic acid were knocked out) were used to produce 2,3-butanediol, in which K. pneumonia HR521 LDH (gene for lactic acid was knocked out) was the best for the production, and then the fermentation medium was optimized by orthogonal design. The optimum compositions were as follows: glucose 90 g/L, (NH4)2HPO4 3 g/L, CLSP 6 g/L, sodium acetate 5 g/L, KCl 0.4 g/L, MgSO4 0.1 g/L, FeSO4 x 7H2O 0.02 g/L, MnSO4 0.01 g/L. Under the above conditions, final concentration of acetone and 2,3-butanediol could reach 37.46 g/L, 10 g/L higher than that under the initial conditions, the yield was 90.53% of the theory, and the productivity was 1.5 g/(L-h), and no lactic acid was detected, which could be benefit for the downstream processing and industrial application.
