In vitro anti-inflammatory and free radical scavenging activities of flavans from Ilex centrochinensis.
- Author:
Lu-jun LI
;
Li-juan YU
;
Yan-ci LI
;
Meng-yuan LIU
;
Zheng-zhi WU
- Publication Type:Journal Article
- MeSH:
Animals;
Anti-Inflammatory Agents, Non-Steroidal;
chemistry;
pharmacology;
Cell Line;
Cyclooxygenase 2;
immunology;
Drugs, Chinese Herbal;
chemistry;
pharmacology;
Flavanones;
chemistry;
pharmacology;
Free Radical Scavengers;
chemistry;
pharmacology;
Ilex;
chemistry;
Interleukin-6;
immunology;
Macrophages;
drug effects;
immunology;
Mice;
Nitric Oxide;
immunology;
Tumor Necrosis Factor-alpha;
immunology
- From:
China Journal of Chinese Materia Medica
2015;40(8):1523-1528
- CountryChina
- Language:Chinese
-
Abstract:
This study was carried out to evaluate the anti-inflammatory and free radical scavenging activities of flavans from flex centrochinensis S. Y. Hu in vitro and their structure-activity relationship. LPS-stimulated RAW 264.7 macrophage was used as inflammatory model. MTT assay for cell availability, Griess reaction for nitric oxide (NO) production, the content of TNF-alpha, IL-1beta, IL-6 and PGE, were detected with ELISA kits; DPPH, superoxide anion and hydroxyl free radicals scavenging activities were also investigated. According to the result, all flavans tested exhibited anti-inflammatory effect in different levels. Among them, compounds 1, 3, 4 and 6 showed potent anti-inflammatory effect through the inhibition of NO, TNF-alpha, IL-lp and IL-6, of which 1 was the most effective inhibitor, however, 2 and 5 were relatively weak or inactive. The order of free radical scavenging activities was similar to that of anti-inflammatory activities. Therefore, these results suggest that 3, 4 and 6, especially of 1, were,in part responsible for the anti-inflammatory and free radical scavenging activity of Ilex centrochinensis. Hydroxyl group at 4'-position of B-ring plays an important role in the anti-inflammatory and free radical scavenging capacities.
