Protective effect of ginsenoside Rg1 again PC-12 cells in OGD injury through mTOR/Akt/FoxO3 signaling pathway.
- Author:
Jie-ping YAN
;
Nong-er SHEN
;
Qiang YE
;
Yong-hui ZONG
;
Qing-xia FANG
;
Liang-zhong LV
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Cell Proliferation; drug effects; Cell Survival; drug effects; Drugs, Chinese Herbal; pharmacology; Forkhead Box Protein O3; Forkhead Transcription Factors; genetics; metabolism; Ginsenosides; pharmacology; Glucose; metabolism; Oxygen; metabolism; PC12 Cells; Protective Agents; pharmacology; Proto-Oncogene Proteins c-akt; genetics; metabolism; Rats; Signal Transduction; drug effects; TOR Serine-Threonine Kinases; genetics; metabolism
- From: China Journal of Chinese Materia Medica 2015;40(8):1554-1559
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protective effect of ginsenoside Rg1 on oxygen-glucose deprivation (OGD) in PC-12 cells, and preliminarily discuss the potential molecular mechanism of mTOR/Akt/FoxO3 signaling pathway.
METHODThe OGD PC-12 cell model was established. The cell viability was measured by MTT assay. After the pretreatment with Rg1 with the concentration of 10, 20, 40 micromol x L(-1) for 24 h, the cell viability was observed. Lactate dehydrogenase (LDH) release, superoxide dismutase (SOD) ac- tivity and malondialdehyde (MDA) level were detected by colorimetry assay. mTOR, p-Akt(ser473), p-Akt(tjr308), Akt, p-FoxO3, FoxO3 in cytoplasm and nucleus, and total FoxO3 protein expression were detected by Western blot assay.
RESULTOGD could significantly in- hibit cell proliferation in 4-24 h in a time-dependent manner. After pretreatment for 24 h, Rg1 (20, 40 micromol x L(-1)) could notably elevate the cell viability and SOD viability and reduce the LDH release and MDA content. Besides, Rg1 also inhibited OGD-induced mTOR and p-Akt(ser473) decreases. After treatment for 6 h, OGD could reduce FoxO3 phosphorylation and promote FoxO3 in cytoplasm. This data suggested that Rg1 could protect PC-12 cell injury through mTOR/p-Akt/FoxO3 signaling pathway.
CONCLUSIONGinsenoside Rg1 could attenuate OGD-induced PC-12 cell injury. Its action mechanism may be closely related to activation of mTOR/p-Akt/FoxO3 signaling pathway.