Research on ursolic acid production of Eriobotrya japonica cell suspension culture in WAVE bioreactor.
- Author:
Hui-hua LI
;
De-heng YAO
;
Jian XU
;
Wei WANG
;
Qiang CHANG
;
Ming-hua SU
- Publication Type:Journal Article
- MeSH:
Biomass;
Bioreactors;
Cell Culture Techniques;
instrumentation;
methods;
Culture Media;
chemistry;
metabolism;
Eriobotrya;
chemistry;
growth & development;
metabolism;
Triterpenes;
analysis;
metabolism
- From:
China Journal of Chinese Materia Medica
2015;40(9):1693-1698
- CountryChina
- Language:Chinese
-
Abstract:
Through scale-up cultivation of Eriobotrya japonica suspension cells using WAVE bioreactor, the cell growth and ursolic acid (UA) accumulation were studied. The comparison test was carried out in the flask and the reactor with cell dry weight (DW) and UA content as evaluation indexes. The culture medium, DW and UA content were compared in 1 L and 5 L working volumes of bioreactor. The orthogonal test with main actors of inoculation amount, speed and angle of rotation was developed to find the optimal combination, in 1 L working volume of bioreactor. DW of the cell growth and the UA content in bioreactor were higher than those of the shaker by 105.5% and 27.65% respectively. In bioreactor, the dynamic changes of elements in the fluid culture, the dry weight of the cell growth and the UA content in 1 L and 5 L working volumes were similar. Inoculation of 80 g, rotational speed of 26 r · min(-1), and angle of 6 ° was the optimal combination, and the cell biomass of 19.01 g · L(-1) and the UA content of 27.750 mg · g(-1) were achieved after 100 h cultivation in 1 L working volume of bioreactor. WAVE Bioreactor is more suitable than flasks for the E. japonica cell suspension culture, and culture parameters can be achieved from 1 L to 5 L amplification.
