A novel trichostatin analogue culture of Streptomyces sp. CPCC 203909.
- Author:
Ming-hua CHEN
;
Ye-xiang WU
;
Yan-ni XU
;
Li-yan YU
;
Bin HONG
;
Wei JIANG
;
Shu-yi SI
- Publication Type:Journal Article
- MeSH:
Cell Survival;
drug effects;
Fermentation;
Hep G2 Cells;
Humans;
Hydroxamic Acids;
chemistry;
isolation & purification;
metabolism;
pharmacology;
Molecular Structure;
Streptomyces;
chemistry;
metabolism
- From:
China Journal of Chinese Materia Medica
2015;40(9):1751-1754
- CountryChina
- Language:Chinese
-
Abstract:
By using a cell-based high throughput screening model for the CLA-1 up-regulator, Streptomyces 203909 was found to produce up-regulator of CLA-1. A novel trichostatin analogue was isolated from the rice fermentation of Streptomyces sp. CPCC 203909by a combination of various chromatographic techniques including column chromatography (CC) over silica gel, flash C18 CC, and reversed-phase HPLC. Its structure was identified as (-)-(R,2E,4Z)-7-[(4'-dimethylamino) phenyl]-4,6-dimethyl-7-oxohepta-2,4-dienoyl-L-glutamine (1) by the spectroscopic and chemical methods, and combination with the CD spectroscopy and Marfey's method. In the prelimi- nary assays, Compound 1 showed cytotoxicity against human embryonic kidney 293 cell line with IC50 value 35.3 [µmol · L(-1).
