Ethyl acetate-soluble chemical constituents from branch of Hypericum petiolulatum.
- Author:
Qi ZHAO
;
Hai-long ZHANG
;
Xue ZHANG
;
Xiao-meng LI
;
Yu-zhen SUN
;
Hang SU
- Publication Type:Journal Article
- MeSH:
Animals;
Antioxidants;
chemistry;
isolation & purification;
pharmacology;
Drugs, Chinese Herbal;
chemistry;
isolation & purification;
pharmacology;
Hypericum;
chemistry;
Lipid Peroxidation;
drug effects;
Microsomes, Liver;
drug effects;
metabolism;
Molecular Structure;
Oxidative Stress;
drug effects;
Plant Stems;
chemistry;
Rats
- From:
China Journal of Chinese Materia Medica
2015;40(9):1755-1758
- CountryChina
- Language:Chinese
-
Abstract:
The stems and branches of Hypericum petiolulatum were extracted by alcohol and liquid-liquid extraction. Seven furofuran lignans were isolated from the ethyl acetate fraction of ethanol extract of H. petiolulatum by using silica gelchromatography, Sephadex LH-20 chromatography, medium-pressure liquid chromatography and preparative HPLC. Their structures were identified by the spectroscopic methods as pinoresinol (1), medioresinol (2), 8-acetoxypinoresinol (3), epipinoresinol (4), (+)-syringaresinol (5), (+)-1-hydroxysyringaresinol (6) and erythro-buddlenolE (7). All the isolates were firstly found in H. petiolulatum. In the bioassay, compound 7 showed remarkable antioxidative activity inhibiting Fe(+2)-cystine induced rat liver microsomal lipid peroxidation with inhibitory rate 38% at a concentration of 1 x 10(-6) mol · L(-1) (positive control Vit E with the inhibitory rate of 35% at the same concentration).
