Study on quality standard of Eucommia ulmoides based on absorbed active components in rat plasma.
- Author:
Xing LIU
;
Xiao-jian GONG
;
Hua-guo CHEN
;
Mei ZHOU
;
Xin ZHOU
- Publication Type:Journal Article
- MeSH:
Animals;
Chlorogenic Acid;
blood;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
analysis;
Eucommiaceae;
chemistry;
Glycosides;
blood;
Iridoid Glucosides;
blood;
Lignans;
blood;
Male;
Plasma;
chemistry;
Rats;
Rats, Sprague-Dawley;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2015;40(9):1771-1775
- CountryChina
- Language:Chinese
-
Abstract:
The method was established for determination of geniposidic acid, chlorogenic acid, pinoresinoldiglucoside, which are three kinds of constituents of Eucommia ulmoides absorbed into the blood components. LC-MS/MS technique was applied to determine the blood components of the bloodstream after administration of E. ulmoides extract. At the same time, HPLC was used for detection of the ingredients content of the blood sample from 23 batches of E. ulmoides. The results showed that geniposidic acid, chlorogenic acid and pinoresinoldiglucoside are prototype into the blood in rats after oral administration of E. ulmoides extract, The linear range of geniposidic acid, chlorogenic acid and pinoresinoldiglucoside was good, and the average recoveries geniposidic acid, chlorogenic acid and pinoresinoldiglucoside were 98.69%, 100.8% and 98.39%, respectively. The method is simple and feasible with good reproducibility.
