Determination of residual organic solvents and macroporous resin residues in Akebia saponin D.
- Author:
Qiao-han WANG
;
Xiao-lin YANG
;
Wei XIAO
;
Zhen-zhong WANG
;
Gang DING
;
Wen-zhe HUANG
;
Zhong-lin YANG
- Publication Type:Journal Article
- MeSH:
Chromatography, Gas;
instrumentation;
methods;
Drug Contamination;
prevention & control;
Organic Chemicals;
analysis;
Reproducibility of Results;
Resins, Synthetic;
chemistry;
Saponins;
analysis;
isolation & purification
- From:
China Journal of Chinese Materia Medica
2015;40(10):1960-1964
- CountryChina
- Language:Chinese
-
Abstract:
According to ICH, Chinese Pharmacopoeia and supplementary requirements on the separation and purification of herbal extract with macroporous adsorption resin by SFDA, hexane, acetidine, ethanol, benzene, methyl-benzene, o-xylene, m-xylene, p-xylene, styrene, diethyl-benzene and divinyl-benzene of residual organic solvents and macroporous resin residues in Akebia saponin D were determined by headspace capillary GC. Eleven residues in Akebia saponin D were completely separated on DB-wax column, with FID detector, high purity nitrogen as the carry gases. The calibration curves were in good linearity (0.999 2-0.999 7). The reproducibility was good (RSD < 10%). The average recoveries were 80.0% -110%. The detection limit of each component was far lower than the limit concentration. The method is simple, reproducible, and can be used to determine the residual organic solvents and macroporous resin residues in Akebia saponin D.
