Protection of Shenlian extracts to PM2.5 infected RAW 264.7 cell damage.
- Author:
Xu-cen LIU
;
Yu-jie LI
;
Ya-jie WANG
;
Qi LI
;
Qing YANG
;
Xiao-gang WENG
;
Chen YING
;
Wei-yan CAI
;
Yan GUO
;
Xiao-xi KAN
;
Xi CHEN
;
He-fei HUANG
;
Xiao-xin ZHU
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Line;
Drugs, Chinese Herbal;
pharmacology;
Macrophages;
drug effects;
Mice;
Oxidative Stress;
drug effects;
Particulate Matter;
toxicity;
Protective Agents;
pharmacology;
Reactive Oxygen Species;
metabolism
- From:
China Journal of Chinese Materia Medica
2015;40(10):1977-1983
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this research is to investigate the protection of PM2.5 infected RAW264.7 cell by traditional Chinese medicine (TCM)--Shenlian(SL) extracts and to establish the damage model. We use cell growth, cell damage and oxidative stress related markers, and inflammatory cytokines as observation index to evaluate the protection of PM2.5 infected RAW264.7 by SL extract. The results showed that 50 mg x L(-1) PM2.5 could cause cell particle deposition, inhibit the growth of cells, and significantly increase the cell supernatant of LDH, NO release quantity and intracellular reactive oxygen species (ROS) level during 4 h and 24 h. In the intervention of SL extract 50, 25, 10 mg x L(-1), the particle deposition of RAW264.7 cells, cell supernatant of LDH, NO, IL(-1) beta release, MCP-1 was significantly decreased, the SOD activity increased significantly. It shows that SL extracts of PM2.5 infected RAW264.7 cell damage has obvious protective effect, the effect may be related to the direct protection of cells, reduce oxidative stress and inflammatory injury.
