Primary study on changes of serum proteomics in rabbit superior mesenteric artery occlusion shock.
- Author:
Hong-Jie LI
1
;
Rui-Li YAO
;
Hui-Li WU
;
Chang-Ying LI
;
Shi-Xin WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Arterial Occlusive Diseases; blood; complications; Aryldialkylphosphatase; metabolism; Blood Proteins; metabolism; Female; Haptoglobins; metabolism; Male; Mesenteric Artery, Superior; pathology; Proteome; metabolism; Proteomics; methods; Rabbits; Shock; blood; etiology
- From: Chinese Journal of Applied Physiology 2011;27(2):206-209
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explor the changes of serum proteomics in rabbits superior mesenteric artery occlusion (SMAO) shock as well as its possible effect in SMAO shock.
METHODSSMAO shock model in rabbits were induced by occlusion of the superior mesenteric artery, serum samples were obtained from rabbits before and after SMAO shock, proteins in samples were separated by two-dimensional electrophoresis(2-DE), spots in the 2-DE map were detected and evaluated by PDQuest software 8.0. The spots with different expression level were subjected to matrix assisted laser desorption/ionization-time of flight-time of flight-mass spectrometry (MALDI-TOF-TOF-MS) for identification, the protein database was searched to further characterized the differential proteins.
RESULTS19 differential protein spots were screened out in the 2-DE maps, 11 proteins were up-regulated and 8 proteins were down-regulated in SMAO shock rabbits' s serum. 4 of the 19 differential protein spots were selected for MALDI-TOF-TOF-MS study, and 2 of the 4 differential protein spots were identified satisfactoryly as paraoxonase and haptoglobin, which content were increased in rabbits' s serum after SMAO shock.
CONCLUSIONSerum proteomics of rabbit change remarkablely before and after SMAO shock, paraoxonase and haptoglobin may be associated with the compensation after SMAO shock.
