The relationship between endoplasmic reticulum stress and its particular apoptosis way caspase-12 and apoptosis in renal cortex of diabetic rats.
- Author:
Yan-Ping CAO
1
;
Yong-Mei HAO
;
Qing-Juan LIU
;
Jian WANG
;
Hang LI
;
Hui-Jun DUAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Caspase 12; metabolism; Diabetes Mellitus, Experimental; complications; Diabetic Nephropathies; pathology; Endoplasmic Reticulum Stress; Heat-Shock Proteins; metabolism; Kidney Cortex; metabolism; pathology; Male; Proliferating Cell Nuclear Antigen; metabolism; Rats; Rats, Wistar
- From: Chinese Journal of Applied Physiology 2011;27(2):236-240
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expressions of 78-kDa glucose-regulated protein (GRP78) and Caspase-12 and their relationship with apoptosis in renal cortex of diabetic rats.
METHODSUninephrectomized Wistar rats were used to induce diabetes by intraperitoneal injection of Streptozotocin (STZ 65 mg/kg). After 8 weeks, the expression and distribution of GRP78, Caspase-12, proliferating cell nuclear antigen (PCNA) were examined by immunohistochemistry. Flow cytometry was used to detect the levels of protein of GRP78 and Caspase-12. Apoptosis was evaluated by means of terminal deoxynucleotidyl transferase-mediated d-UDP nick-end labeling (TUNEL) and Flow cytometry. Serum creatinine, blood urea nitrogen and 24-hour urine protein excretion were checked.
RESULTSCompared with those in normal control group, the numbers of apoptosis and the expression of GRP78, Caspase-12 in glomerular and tubular cells were much higher in the diabetic kidneys at 8 weeks. There was no significant difference between group A and group B.
CONCLUSIONActivation of endoplasmic reticulum stress may play an important role in the development of diabetic nephropathy.
