Development and application of the physical hypoxic models of C. elegans.
- Author:
Chang-hong REN
1
;
Ji-ye ZHANG
;
Jin-ping SHI
;
Bin JIANG
;
Na ZHAO
;
Hu-qi LIU
;
Cheng-gang ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Caenorhabditis elegans; physiology; Caenorhabditis elegans Proteins; metabolism; Disease Models, Animal; Hypoxia; physiopathology; Hypoxia-Inducible Factor 1; metabolism; Neurons; pathology; Transcription Factors; metabolism
- From: Chinese Journal of Applied Physiology 2011;27(3):257-262
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a suitable hypoxic injury model, which is important for revealing pathological molecular mechanism of hypoxia.
METHODSWe focused on C. elegans by treatment with different hypoxic times and systematically observed mortality, movement, Cellular morphology and the related-protein expression of the animals.
RESULTSWe demonstrated that hypoxia (0.2% partial pressure of oxygen) induced morphological cell defects, and then leading to death of C. elegans. The mortality of C. elegans increased along with hypoxic time, while hypoxia-inducible factor (HIF-1) was significantly up-regulated. In addition, by using neuron-specific transgenic wonns with green fluorescent protein--we observed the neuron-specffic injury caused by hypoxic stress.
CONCLUSIONWe successfully established an effective, convenient physical hypoxic model of C. elegans, which will facilitate the studies of hypoxic pathology and molecular mechanisms of hypoxic response in the future.
