The effect of genetic polymorphism of the exon 2 of the beta-lactoglobulin gene on the milk composition in Chinese Holstein.
- Author:
Fan YANG
1
;
Xue-jun ZHOU
;
Nan-qiao LIU
;
Yu ZHANG
;
Guo-liang WU
;
Yue CHENG
;
Xi-wen HUANG
;
Ya-fei CAI
;
Gen-lin WANG
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Animals; Base Sequence; Cattle; classification; genetics; China; Exons; Female; Lactoglobulins; genetics; Milk; chemistry; Molecular Sequence Data; Polymerase Chain Reaction; methods; Polymorphism, Single-Stranded Conformational
- From: Chinese Journal of Applied Physiology 2011;27(3):333-337
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEIn order to study the effect of the polymorphism at the exon2 region of the (3-LG allele gene on milk composition and yield.
METHODSThe single-strand conformation polymorphism method (PCR-SSCP) was used to analyze for polymorphism the exon2 region of the 3-LG gene (NCBI accession number: DQ489319) in Chinese Holstein.
RESULTSEight SSCP patterns were detected in the fragments: ab, abc, abd, abe, abcd, abce, abde and abcde, and the patterns frequencies as follows: 0.14, 0.10, 0.27, 0.23, 0.05, 0.04, 0.11 and 0.06 (P < 0.05); Six single nucleotide polymorphism (SNP) were detected in this study: sitel C>T, site2 T>C, site3 C>T, site4 C>C, site5 C> A, site6 A>T or C, and the polymorphism infonnation content (PIC) of these SNPs were in median or high polymorphism (PIC > 0.25).
CONCLUSIONThese SNPs at the exon2 region of the beta-LG gene were remarkably and affected milk performance traits (milk yield, protein and fat contents) in Chinese Holstein.
