Changes of GABA-activated currents in isolated dorsal root ganglion neurons in rats with neuropathic pain.
- Author:
He ZHU
1
;
Ke-tao MA
;
Li LI
;
Zhong-shuang ZHANG
;
Jing LI
;
Jun-qiang SI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Separation; Constriction; Ganglia, Spinal; pathology; physiopathology; Male; Neuralgia; etiology; physiopathology; Neurons; metabolism; physiology; Patch-Clamp Techniques; Rats; Rats, Sprague-Dawley; Receptors, GABA-A; metabolism; physiology; Sciatic Nerve; injuries
- From: Chinese Journal of Applied Physiology 2011;27(3):376-379
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changes of GABA-activated currents in isolated dorsal root ganglion neurons in rats with neuropathic pain.
METHODSThe neuropathic pain model was established by chronic constriction injury (CCI) 7 days before electrophysiological-recording. The rat DRG neurons were enzymatically dissociated. Whole-cell patch clamp technique was used to record GABA-activated currents. The changes of currents of injured side and opposite side were expected to compare with control group.
RESULTS(1) The currents of injured side of CCI group were notablely decreased compared with control group (GABA concentration, 0.1-1000 micromol/L). (2) By the contrast, opposite side currents of CCI group increased significantly compared with those in injured side and control group (GABA concentration, 0.01-1000 micromol/L).
CONCLUSIONThe data indicates that the chronic constriction injury change both the function of GABAA receptors of injury side and opposite side. The decrease of pre-synaptic inhibition of GABA may be the possible reason of neuropathic pain.
